How do I better visualize protein interactions at edges of periodic box in VMD?

I have a 500 ns molecular dynamics trajectory with several proteins in a membrane. The entire system is enclosed in a periodic box. Unfortunately, my proteins are only interacting around the...

11 November 2020 6,912 3 View

If I Have one Group of Participants, is There a Statistical Analysis for this?

5 August 2020: Looking for assistance on a review of the calculation of my work on Kendall's W and Tau. This method made more logical sense in that its heavily used for survey responses. I also...

27 June 2020 5,983 4 View

How expensive are commercially available customized molecular dynamics services?

I am interested in potentially using commercially available customized HPC molecular dynamics simulations. However, I cannot find pricing information on the websites of the vendors. Could someone...

07 June 2020 9,137 3 View

Does EDC/NHS coupling act on primary amides or only primary amines?

I want to use a coupling reagent (possibly EDC/NHS) to link gold nanoparticles to primary amines and NOT primary amides. There are both primary amines and primary amides in my sample, but I want...

16 January 2020 7,378 4 View

Protein dialysis of extremely hydrophobic outer membrane protein to prevent precipitation?

I am currently trying to run dialysis on a protein purified using the ProBond Ni-Ta purification columns against a buffer of 10mM TRIS (pH 8.0) and 0.1% Triton X100 in sterile millipore water. My...

14 February 2019 1,660 14 View

What freeware would you recommend for tracing neurons in a tiff stack?

I've acquired a stack of tiff images from a volume of brain tissue. I'm trying to find an automated program (not semiautomated) which will allow me to trace the neurites within this volume.

19 September 2018 1,332 1 View

What is the "pore size" of brain tissue?

I was wondering about the ECM "pore size" within rat brains (in the context of nanoparticle diffusion). In addition, I would like to know if the fixation/treatment method impacts the tissue's...

15 June 2018 6,349 9 View

Linking User Subroutines in Abaqus 2018?

Hello, I am modeling a complex process and I need to use a user subroutine to so. I am using the newest version of Abaqus and I cannot seem to get the compiled subroutine linked with the solver....

24 May 2018 8,901 11 View

Primers once worked for QRT-PCR but now not amplifying my gene of interest.

06 February 2017 4,130 1 View

How many simultaneous proteins can be analyzed within a single Western Blot? Is the same true of RT-PCR?

I am beginning a new research project and trying out Western Blotting for the first time. I plan to analyze the protein expression of EAAT-2, mGlur5, NMDA-NR2B, and a few other proteins...

19 July 2013 1,350 5 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View