What do you want to observe or measure in the animals? This will determine what amount of juice you can or must give them.

I wanted to measure total cholesterol and MDA sir.

I assume that you have a good hypothesis to test or a logical question to answer by this trial. That will determine how to set up your trial.

If such a hypothesis might be absent and the question might only be, just see what happens, any trial might well be a (large) waste of time, money and resources. 

In my country, any animal trial has to be approved by an Animal Ethics Committee. So if you do not provide them with a firmly based plan, you will not get approval to carry out the experiment.

Hi,

You need to carry out acute toxicity study to determine the accurate dosage levels. This in turn referes to strong hypothesis to get approval from Animal Ethical (or Ethics) Committee.

However, you may refer to available literatures to determine the dosage you want to test for some parameters. Generally, it ranges from 100 mg/Kg to 1000mg/Kg. Because it may vary with herbal species or type of extract, you need to search the literature in terms of the general toxicity associated with particular higher dosage (enabling strong hypothesis).

Hope this help.

Thank you Mr.Patil. The Journal about Acute toxicity dose is already available. But in this case, it's an extract. Therefore can I use the extract acute toxicity as a reference to determine my juice dosage?

Thank you, Mr Lavekar. Is there any reference that I can use? Much obliged. 

Use acute toxicity test , I have done with flower extract drink.   Doses are vary,depend on which test you carry on. Extraction also is important. Normally it will base on the ratio of  Vista rat  weight /concentration of extraction from herbal juice.

Generally 20 ml hebal juice is used for therapeutic benefit  in human but for study the therapeutic claim on scientific parameters in rats, we should go for dose finding study at different dose level and after that we select the dose having highest therapeutic efficacy.  We should also specific about the method of preparation of herbal juice, specific method have specific dose

Herbal juice is the form of Swarasa Kalpana in Ayurvedic Pharmaceutics. Normally when we go for dose conversion from human into rats we have applied Paget and Barnes rule 1964. The human dose of Swarasa (herbal juice) is 24 ml.

Thank you, Mr Singh, Mr Yadav, and Mr Srichaikul for the knowledge and answers. Much obliged