I am working with proteins, using 2D, western blotting techniques. I want to detect phosphoproteins in saliva. So kindly help for me how to simply detect and analysis the salivary phosphoprotein. Anyone tell me please.
Thank you,
Probe your membranes with anti-phosphoSer, phosphoThr, or phosphoTyr antibodies.
Refer this paper.
Detection, Localization and Tyrosine Phosphorylation Status of Ser/Thr Protein Phosphatase1γ in Freshly Ejaculated, In Vitro Capacitated and Cryopreserved Buffalo Spermatozoa
ARTICLE in REPRODUCTION IN DOMESTIC ANIMALS 50(6) · OCTOBER 2015 with 4 READS
Impact Factor: 1.52 · DOI: 10.1111/rda.12598
Hi,
I'm not sure what your question is, do you want to know WHY we study phosphoproteins (refer to Md. Moniruzzaman) or HOW to study them?
As for protein extraction from saliva, refer to the attached paper. Once proteins have been extracted (in the presence of phosphatase inhibitors) you can follow a standard western blotting protocol for phosphoproteins such as the one attached from abcam.
good luck
John
Article Purification and characterization of a rabbit salivary prote...
dear researchers: i want to know if any non fluorescent staining method for phosphoprotein detection.
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