Hi, I'm looking to derivatise sulphated monosaccharides and disaccharides for GCMS. Normally I use pyridine-MSTFA but it doesn't seem to work for the monosaccharide (Galactose-4-sulphate sodium salt).
Is there a way to derivatise them directly for GCMS? It's okay if the sulphate group doesn't show a unique derivatisation when compared to a carboxylic or hydroxyl group.
I would try silylation. I have had great results, easy to do, water is the problem but lyophilization is the solution.
Hi Ernesto,
GCMS seems to work well for neutral sugars, but the sulphated ones are giving me problems in that they are not derivatising well, or the sulphate keeps them from volatilising.
If you have tried GCMS of sulphated sugars, please let me know the compounds you have tested and how the results went.
Thanks.
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