Hi all
This is new territory for me. I'm a mammalian cell culture person but recent research has driven me into using new organisms. I have started working with Anabaena (D flos aquae) and after observation under a microscope and what I have seen I know they grow in filaments but authors that have worked on this organism mention the cells are plated often 10^4 cells/m without specifics of how the cell counts are carried out or estimated. The problem is how do you count these cells in the filament which is very tiny under the microscope to estimate the number of cells in 1 ml. The question may sound stupid but it's just new territory for me as I have said. Thanks in advance for your contributions.
Hi. Green algae can be counted using 4 major ways, please read this:
Quantitative Determinations (wisc.edu)
I usually counted them under a epifluorescence microscope, using small drops of liquids. dead cells would appear brown while live cells would appear glowing red.
You can also use a hemocytometer to plate a small volume and count :)
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