Dear Tina,

I am not surprised that you can't convert an Excel file into an other format such as .meg or .fasta. What kind of data do you have in your excel file? Is it just a list of sequences to align?

If yes, you can open a note pad file (.txt) and you can copy/paste your sequences following a fasta format :

>Individual1

ATGCATGCATGCATGC

>Individual2

ATGCATGCATGCATGC

>Individual3

ATGCATGCATGCATGC

etc.

You save your file and then you change its extension .txt by .fasta. Normally, MEGA is able to read such a file.

NB. Be careful if you copy and paste several sequences from Excel to Note pad, they usually add quotation marks (") that you will have to supress before doing your analysis.

I hope this answered your question...

Dear Dr Guillaume Tetreau,

Thanks a lot for your response.

But this is how my input data file looks like in .xls:-

Sample Population Loci 1 Loci 2 Loci 3

SBH001 Short tail 277 279 148 148 156 159

And I have got 100 samples and 20 microsatellite loci. The numbers 277/279 denotes allele size in base pair.

Do you think it can be converted into .fasta?

Thanks again.

Cheers,

Tina