For drugs, we can use the UV Vis spectrophotometry in permeation experiments, but how can the permeation of particles be checked? (Particles are soft, around 200-400 nm)
Soft nano-particles? Are you studying inorganic solid [engineered] nano-particles as found in lotions? I presume, you are interested to study the blocking of permeation of nano-particles. Or are you interested in studying translocation of nano-particles in the body and how this process can be arrested [that leads to treatment] such that the symptoms of disease states related to nano-particles subside as a result? There are nano-biotech solutions for bio-chelation that bind nano-particles for safe removal through urine, in which case, the nano-particles may be studied in urine samples. Some additional info may be helpful.
Confocal microscopy with fluorescent nanoparticles, the FL label has to be covalently linked to the carrier. IMHO particles with 200-400 nm size can at most populate the hair follicles/ wrinkles in the skin, don't expect them to reach dermis.
Better way you can use the permeable membrane, which resembles the skin. Then by using physiological buffer you can measure how much amount of nano-particles crossed that membrane.
Thanks Shreya for coming out with the question. Nilesh can you advise what type of membrane do you mean? can you provide specific example or any article related to it. thanks
Depends what study you want to carry on?
If you want to check whether the drug loaded in nanoparticles permeated or not? You should go for skin perfusion experiments.
If you only want to assess whether your prepared NPs are permeating through skin than fluorescent labelling will help.
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