How do I calculate polypeptides pHi, especially when we have repeated amino acid?

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How i can activated Strain rate effect for solid65 element in ANSYS -APDL ?

05 May 2023 3,750 1 View

I am looking for 3d simulator that can help me similate activities in smart home environment and generate a dataset based on what the individual doin?

what the individual is doing inside this smart home ( the collection of the dataset is based on the individual and its interaction with sensors in this smart home)

23 February 2023 2,343 0 View

Can physical techniques such as ultrasonic bubbles generation be used to form high porosity pastes ?

We want to obtain highly porous pastes without resorting to chemical methods such as the use of expanding agents.

13 January 2023 1,400 1 View

How do I draw the curve lateral load-displacement curve?

hello everybody i am trying to study the behavior of RC shear walls under cyclic and monotonic loading using ANSYS APDL. How do I draw the curve lateral load-displacement curve? how i ca import...

10 June 2022 5,851 2 View

How much time can we conserve ewes blood serum samples in - 20°C for biochemical analysis?

Hello, I would like to know how much long can I conserve serum samples in - 20°C for biochemical analysis : lipid profile (cholesterol, triglyceride...), protein profile (albumin, total...

29 May 2022 9,295 15 View

Pelvic opening might be considered as a criteria in goat genetic selection programs?

Looking for researches or documents which have already included the pelvic opening in goat as a criteria in selection programs. Thank you in advance

13 January 2022 9,757 1 View

UAMP for function reaction force and loading amplitude ???

Hell evry bdy e,I am using the UAMP routine to apply the axial load in the node relatively from the sensor value that corresponds to the date output on each increment. I am using coding to...

04 May 2021 6,891 0 View

What are the criteria for selecting variables for PCA analysis ?

How can I select variables for PCA analysis from huge set of data? except the biological significance of the variables, are there other criteria for objectively choosing the most relevant...

21 February 2021 9,757 10 View

Does ph-meter specific for virology exist ?

Hi everyone, Currently i am working to Norovirus and SARS-Cov -2 assays, I would like to know if a ph meter specific for virology exists ? I mean that we need to test pH from several samples...

09 September 2020 1,776 3 View

Which is the most appropriate graphical method to represent distances between breeds concerning one locus?

I'm studying the polymorphism of a microsatellite related productive performance in 4 goat breeds and I would like to represent the distances between them according to its polymorphism (alleles...

05 February 2020 1,810 3 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How can AI technology to enhance the agricultural productivity?

Farmers no longer have to apply water, fertilizers, and pesticides uniformly across entire fields. Instead, they can use the minimum quantities required and target very specific areas, or even...

22 July 2024 8,296 3 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

How can the resilience of agricultural systems be improved by both gradual climate change and increased climatic variability and extremes?

17 July 2024 3,681 3 View

Robert James McClelland

For repeated measures your posthoc (if needed) would be for equal variances not assumed (take your pick from Dunnet's, Tamahane's or Games-Howell tests)

On the process for general approach, you may find following example useful:

https://statistics.laerd.com/statistical-guides/repeated-measures-anova-statistical-guide.php

Jukka Salminen

Try this calculator (at your own risk):

http://isoelectric.ovh.org/

Hossem SAHRAOUI

Thank you Robert, thank you Jukka