Hi there~~

You can't calculate Fo' and Fm'. You can measure them. The principles of the all PAM fluorometers are same. Please look at the attached paper. I hope it will be helpfull. If something still will be unclear you can message to me. Don't hesitate.

Good luck~~~

Sincerely,

Ismayil

Thanks for your kindly Help

Hello, Faride, the only difference between Fm, Fo and Fm', F' and Fo' are the conditions under which the sample is measured. The apostrophe ( ' ) indicates some point after light induction of photosynthesis, usually steady-state under the light. If I am not mistaken the Handy PEA does not have the capacity to excite the sample under lit conditions, which would be a requisite of taking standard measurements of Fm' and F'. Fo' requires cessation of light after induction to measure the Fo' under photosynthetic conditions.

Hello,

yes. unfortunately I don't have any data about Fo', Fm' (Chlorophyll fluorescence parameters in lightness). My data is in the attached file.

Thanks for your attention.

Yes, it appears you won't be able to get F' or Fo' with that instrument. But that may not be a problem. It appears that you could look at OJIP variables , which would be very useful. I suggest the review by Stirbet and Govindjee Article On the relation between the Kautsky effect (chlorophyll a fl...

Thank you so much.

you can't have these parameters with that instrument maybe with the OJIP or Handy PEA u can