Hello Opeyemi,

Unfortunately, I'm not clear on what you're asking. Can you possibly describe in a bit more detail what variable/s you have, what analysis or analyses you have run or wish to run, and, most important, what specific research question or hypothesis you are trying to evaluate?

Good luck with your work.

I agree with David Morse. There is a need for some clarifications on the tests you are running. However, you can get your means, standard deviation, Count and SEM on Excel. Then you take the Standard deviation or SEM into any statistical tool/package of interest. That is when you will define your level of significance, either 95% (p

You can do t test for mean as a one sample and find p value.

I measured p by different way

It depends on your sample size. Sometimes your triplicates do not need SEM to calculate the P-value (significance), you can use T-test to calculate p-value. the fist thing you should understand about your data is if your triplicate variance is equal or not equal to your control. you need to do the F-test to measure that. then you can use the T-Test with either equal variance or unequal variance (based on the F-test). If your triplicates is before and after experiment the they are the same group, if not you should use Two Sample T-sets.

The SEM, is if you have different populations (your triplicates of a single experiment should be repeated many times, lets say six times; each set should have their own means), then you measure the SD of each, then you measure the SD of the different means of the different experiment set and that's the SEM.