How do extract a twitter conversation thread?

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Is this a mixed methods approach?

For my PhD dissertation: If Chapter 1 is a systematic literature review (QUANTS/QUALS) while Chapters 2, 3, and 4 are empirical chapters (QUALS), is this considered a mixed methods research...

16 June 2024 3,220 5 View

In the case of Collagen Cell Carrier® membrane 34 mm for 6 well cell culture plate (Viscofan), can you suggest a paraffin embedding protocol?

I am intrested in the preparation of collagen carriers, dehydration, paraffin infiltration, and paraffin embedding. Thanks in advance.

05 June 2024 3,831 0 View

Mentorship and Collaboration in Medicinal and Organic Chemistry ?

I am looking for for mentor in medicinal or organic chemistry to help guide me in my research and career development. I am also open to collaborating with researchers who share my interest. If...

26 May 2024 5,186 2 View

How can I detect a small amount of Protein without overexpression?

Hi, are there other methods beside Western Blotting to detect a specific protein? I‘ve isolated mitochondria and want to show that hPar17/Pin4 is present in mitochondria. The Western Blots didn’t...

24 May 2024 5,500 3 View

How many keratinocytes and fibroblasts are contained in 1 mm^2 of skin?

Good afternoon, everyone. Perhaps someone knows how many keratinocytes and fibroblasts are contained in 1 mm^2 of skin. Or the ratio of these cell types may be known. If there is a link to an...

15 May 2024 5,256 0 View

Which antibodies can be used to detect mitochondria in western blots?

Hi! I´m currently trying to isolate Mitochondria from HCM cells. For my western blots I need an additional antibody against a mitochondrial protein which is bigger or smaller than 14/17 kDa. I was...

24 April 2024 8,814 2 View

How do I block mouse Fc receptors when my primary antibody is raised in rat?

Hi, I have having an issue with the Fc blocking stages when carrying out flow cytometry on a murine macrophage cell line. I am using a rat anti-mouse antibody against the scavenger receptor...

22 April 2024 3,466 2 View

Thematic analysis or grounded theory?

i am currently conducting a critical appraisal of a research article and the data analysis is explained as: interviews transcribed verbatim and participants were given the option to review and...

21 April 2024 1,821 7 View

Does anyone have ultrasonic audio files of male mice?

For a project I'm included in I'm desperately in need of audio recordings of male mice. They need to be original files recorded with the correct equipment. I need them for research on gait...

19 March 2024 4,959 1 View

How lower year interacts with higher year students in America?

lower year students attitude towards higher year students in America

09 March 2024 4,747 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

In WECS : Gain values for conversion of PMSM rotor speed(rad/s) to p.u. input to wind turbine and Torque(pu) of WT to input of PMSM-Torque (Nm) ?

I am working on developing a MATLAB Simulink model of Wind turbine with PMSG followed by MPPT Boost converter. I have gone through so many literature and videos but I am not finding clear solution...

03 August 2024 888 1 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

How can I extract my bibliography from researchgate ?

how can I extract my bibliography from researchgate ?

28 July 2024 6,737 1 View