How do different forms of oligomycin alter cellular metabolism?

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Rafael Franco

Hi Eóin

As you place the question the answer would be. Not needed if you achieve total inhibition of ATP synthase using oligomycin A. But perhaps you are not achieving total inhibition?

Eóin O'Brien

Hi Rafael,

I have been using the oligomycin with a Seahorse analyser so it is hard to tell if it is completely gone. It seems to inhibit oxygen consumption quite well however I do not seem to be getting the expected increase in extra cellular acidification which is why I thought there may be another one required.

Thanks