Eóin O'Brien

4 Questions 5 Answers 0 Followers

Questions related from Eóin O'Brien

How do different forms of oligomycin alter cellular metabolism?

We are currently using oligomycin A to inhibit ATP synthase while looking at oxidative phosphorylation however I am wondering if we should also be using other oligomycins (B, C etc) in order to...

12 December 2016 1,272 2 View

Can anyone advise on the effectiveness of osmotic mini pumps (inserted subcutaneously) at increasing the serum concentration of a protein in mice?

We are currently working with NSG mice which are deficient in complement C5a. This protein is important for our disease model but due to it's short half-life repeated injections would be required...

09 September 2016 7,297 2 View

What cells are CD14lowCD16low when gating on whole blood CD14+ cells?

Monocytes are divided into 3 subsets based on their expression of cd14 and cd16 by flow cytometry; Classical(CD14++CD16-), Intermediate(CD14++CD16+) and Non-classical(CD14+CD16++). When I gate on...

01 January 2015 1,511 10 View

Can the THP-1 monocyte cell line be placed into the same subsets as primary monocytes i.e. classical, intermediate and non-classical monocytes?

Classical monocytes are characterised as CD14++ CD16-, Intermediate monocytes express CD14++CD16+ and Non-classical monocytes are characterised by CD14+CD16++ expression.

04 April 2014 9,950 2 View