Huntingtin protein regulates the transcription of BDNF in the cerebral cortex and disrupts the delivery of its to the striatum. Is there any specific way to measure the BDNF in in-vitro culture cell?
Two methods, western blot analysis and ELISA can be used for the quantification of BDNF protein.
For protein quantivication I also recommend ELISA, for quantification of BDNF transcripts I recommend quantitative RT-PCR. However, with array technology you will be able to detect a lot of other transcripts ;-)
For the quantification of BDNF levels in brain tissue (mouse and rat) I would also recommend ELISA. However, if you work with cell cultures, WB and ICC analysis are also possible.
I agree with the previous answers. Currently, we employ WB, RT-PCR and IHC for hamster brain. Contemplating ELISA...
I guess it depends on what you are looking for. ELISA possibly will provide the best protein quantitation in homogenates. RT-PCR is a sensitive technique too, but since it measures mRNA, it provides info about gene expression, WB is not so accurate for quantitation, but provides info about the possible different molecular variants (eg pro-BDNF vs BNDF proper) and may uncover the presence of aggregates (eg dymers). IHC adds info about location within the brain or even subcelluar location...
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