Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Software for flow cytometry-like gating of data sets?

I am currently trying to do image based cytometry from fluorescence microscopy images. From the images I extract data such as cell size and a number of fluorescence intensities. Is there any...

09 February 2021 6,278 1 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Co-stain Lectin PNA FITC conjugate with an uncojugated primary antibody?

Hello, I am planning to stain retinal tissue sections for IHC and want to co-stain cones and rods. For this we plan on using a Lectin PNA From Arachis hypogaea (peanut), FITC Conjugate for cones...

12 January 2021 583 1 View

How to use Abaqus USDFLD subroutine to vary stiffness?

Hi I have a problem where I am trying to vary the stiffness spatially across a structured interface. The whole idea is to vary the stiffness vertically, with a bulk stiffness of 8GPa decreasing...

06 January 2021 262 3 View

I really need help with cov-2 pseudotyped lentivirus production. Support?

Hi, i'm desesperate. i'm trying to produce lentivirus usinf BEI resources kit NR-52948. 24 hours after transfection i detect a massive GFP expression in my 293T cells. After 72 hours i filtered...

30 December 2020 9,889 6 View

How can i estimate the total phenolic content from a plant extract?

Suggestions and recommendations are welcome. A protocol used and experience would also be helpful.

25 December 2020 9,807 4 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

What is the effect of SDS running buffer concentration on western results?

I am trying to do a thermal shift assay for a recombinantly-produced membrane protein, by western blot band quantification. I use Bio-rad 10X Tris/glycine SDS running buffer for my SDS-PAGE and...

11 January 2021 5,302 2 View

How to quantify total protein in crooked western lanes?

I've been using Revert700 total protein stain to detect total protein in my western lanes. While this stain is robust & reversible, I have trouble quantifying in Image Studio Lite (LiCor) and...

05 December 2020 9,014 3 View

Why does immunofluorescence but not western blot works for fusion protein?

I have a flag-tagged protein and want to be sure it is properly expressing in my cells. When I assess by IF, I see clear nuclear enrichment (expected). But when I do a western, there is only a...

27 November 2020 2,350 3 View

Could you kindly assist with the journals that are related to my title below?

Effects of covid-19 pandemic on micro companies within the event sector in Western Cape.

08 November 2020 2,138 2 View

How to increase the signal of the protein standard when performing western blot?

Hi, I'm writing my master's thesis on the characterization of human proteins which I express and purify from E. coli. I'm performing western blot to verify my protein, but the protein standard...

06 October 2020 1,107 5 View

Why am I getting multiple bands in western blot (in Isolating adhesion complexes) ?

Hello everyone, I am isolating adhesion complexes and wanted to confirm the presence of a specific protein in the isolated complex. When I performed western bott, I got a band at the right size...

19 September 2020 538 7 View

How I explain the increase both of the p-AKT and AKT in the western blot?

Hi, I would like to ask a question, the cell treated with drug for 48h, and the cell viability was changed using cck-8 kit, so I think the p-akt is decreased in western, however, the p-akt was...

07 September 2020 4,880 4 View

Can someone point me towards a commercially available E1b19K antibody against Human Ad5-E1b-19K?

#antibody #western #Ad5 #E1b19K

16 August 2020 9,283 1 View

Anti-His Antibody binding everything on a western?

Hello all, I've been expressing some His-tagged proteins in E.coli that appears to be going just fine based on how the SDS-PAGE gels look, so I wanted to confirm with a Western. I transferred my...

22 July 2020 5,255 5 View