Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

03 February 2021 9,311 5 View

When sequencing a plasmid to confirm transformation has been successful, would I have to sequence the full plasmid?

I have transformed some DH5alpha cells with point mutated proteins placed in pcDNA plasmid and extracted the plasmid; now I have to send the plasmid for sequencing for confirming the presence of...

19 January 2021 5,842 3 View

How can decrease the fluorescence background for Raman Spectroscopy?

Could you help me with, how can I decrease the fluorescence background for Raman Spectroscopy?

18 May 2020 7,685 11 View

What are the differences between Raman spectroscopy of amorphous and crystalline carbon materials?

What are the differences between Raman spectroscopy of amorphous and crystalline carbon materials?

30 April 2020 3,435 8 View

How to separate the Raman spectroscopy peak?

In the result of the test for Raman spectroscopy, there are many broad peaks that including a lot of information. In fact, How can I correctly separate those peaks?

26 December 2019 3,460 3 View

What is the best way for poor Nations to conserve thier water resources especially with the current trends of climate change?

I am developing a research on the link between poverty, natural resources management and climate change in Nigeria and need some guidance.

11 December 2016 7,577 0 View

Can i use b-actin in real time PCR?

can i use b-actin as control gene in real time PCR? because in articles that i have, they use GAPDH as a control for real time pcr and b- actin for western blot. i do not know why and what is...

01 May 2016 5,781 10 View

What are the basic fertility destructive factors of tropical soils?

What are the basic fertility destructive factors of tropical soils? Temprature; Effluents; Compaction?

02 March 2016 8,868 17 View

What is the most sustainable way of managing the problems of sawdust and wood shavings accumulation in our cities?

Biological? Physical, or chemical?

07 August 2015 9,700 3 View

Can exploratory factor analysis be performed on a multiple choice question?

10 March 2015 5,728 11 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View