What is the concentration of salts and other materials used in the column binding phase and the washing and release phase؟
Dear Mohsen, many thanks for asking this important technical question which is certainly of interest to everyone working in the field of DNA extraction. For a good overview about this topic please have a look at the following useful guide entitled:
A Complete Guide to How Nucleic Acid Extraction Kits Work
https://bitesizebio.com/13516/how-dna-extraction-rna-miniprep-kits-work/
Pkease also note that there are a few closely related earlier questions available on RG. It might be worth checking out the answers given to those questions:
How do silica based RNA spin columns only bind RNA and not DNA?
https://www.researchgate.net/post/How_do_silica_based_RNA_spin_columns_only_bind_RNA_and_not_DNA
(14 answers)
and
Its possible use spin columns of a DNA extraction kit, in a purification kit?
https://www.researchgate.net/post/Its_possible_use_spin_columns_of_a_DNA_extraction_kit_in_a_purification_kit2
(3 answers)
I hope this helps. Good luck with your research and best wishes, Frank Edelmann
Pls have a look to this link you will find the answers there.
https://www.researchgate.net/post/How_do_silica_based_RNA_spin_columns_only_bind_RNA_and_not_DNA
What is the propse concentration of salts in lysat and other materials used in the column binding phase and the washing and release phase؟ to extracting animal tissue.
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