The following steps can be followed:

Sample Preparation: Crop samples need to be collected and prepared for DNA extraction. The DNA can be extracted using commercially available kits or by a standard laboratory protocol.

Primer Design: The next step is to design specific primers that will anneal to the target DNA sequence. These primers are short DNA sequences that are designed to bind to the complementary regions on either side of the target sequence. The primers should be specific to the target sequence to avoid amplification of non-target DNA.

PCR Amplification: The PCR amplification reaction consists of a reaction mixture that includes DNA template, Taq polymerase, dNTPs, and the specific primers. The reaction is then subjected to a series of temperature cycles that include denaturation, annealing, and extension steps. This process results in the amplification of the target DNA sequence, which can be visualized using agarose gel electrophoresis.

Detection: After PCR amplification, the product can be detected using different methods such as agarose gel electrophoresis, fluorescence, or DNA sequencing. Agarose gel electrophoresis can be used to detect the amplified product by separating the DNA fragments by size and visualizing the bands on the gel.

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