I need to know the conditions of storage isolated mitochondria with the highest activity.
Also, the suitable time to work on it without significant damage.
Hello Nourhan S. Elkholy
Please refer to the book below. It will be very much useful for your study.
PRACTICAL MITOCHONDRIOLOGY Pitfalls and Problems in Studies of mitochondria with a Description of Mitochondrial Functions
by Alexander V. Panov
https://www.researchgate.net/publication/269763545
Also refer to the links below.
https://www.sciencedirect.com/science/article/abs/pii/0076687979550046
https://www.nature.com/articles/4402035.pdf
Good Luck.
Malcolm Nobre
Thanks a lot for your kindly help. They really will be very useful.
Some storage buffers and storage conditions can be found in the links below.
https://wiki.oroboros.at/index.php/O2k-mitochondrial_preparations
Isolated mitochondria should basically not be stored whenever possible.
Even storage on ice inactivates activity extremely quickly.
It is better to perform the experiments immediately after they are isolated to obtain reliable results.
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