Hi everyone,
I have extracted RNA several times with SV total (Promega), Triazol and Triazol followed by SV total.
The Promega kit and Triazol followed by Promega kit, those 2 ways gave fine purity, not degraded RNA on gel but very low concentration (60 and 70 ng/μl), and Triazol gave fine purity and very high concentration but degraded RNA on gel (Tall Smear and not clear 2 bands).
My Questions are:
1. Which way is best for high purity, concentrated and not degraded RNA?
2. What should I do to get high concentration of RNA?
3. Actually I prefer Triazol, how I can get not degraded RNA?
Thank you.
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