How can we search a protein from databese that has low similality to the query enzyme but has the same activity ?

More Yasuhiro Mihara's questions See All

How can we decide the spinning condition when spinning core-shell electrospun fiber?

Dear all, I'm trying to fabricate core-shell electrospun fiber using 5wt%PCL in HFIP as a core solution and 6.5wt%Gelatin in HFIP as a shell solution. Feed rate of core is 0.4 mL/hr (20G) and that...

12 April 2024 7,046 0 View

How best to freeze mouse skeletal muscle for staining of enzyme activity in sections?

Hello, everyone. I would like to determine the fiber type of each skeletal muscle fiber based on its SDH and ATPase activity.I used the dry ice-hexane method to freeze freshly isolated skeletal...

09 April 2022 9,939 2 View

Do you know of any articles on optimizing the powder production from liquid samples using the Taguchi methods?

Dear all. I am interested in the powderization process for liquid components using spray drying and freeze drying. There are quite so many factors involved in such processes, and optimization of...

21 February 2022 8,967 6 View

How can I obtain seeds of temperate terrestrial orchids?

Dear everyone. In addition to my own primary field of research, I am also engaged in research on germination of poorly germinating terrestrial orchids and their breeding as a hobby in life.I have...

26 December 2021 6,031 5 View

What is the most appropriate media for TNFa-treated HUVECs ?

I am Yasuhiro Endo , a medical doctor in Japan. I am going to incubate HUVEC with 10 ng/ml tumor necrosis factor-α (TNF-α; R&D systems, Abingdon, UK) or PBS as control for 5hrs and measure...

06 December 2021 4,138 0 View

Would you tell me how to dissolve cholesterol in RPMI medium?

We are planning to add cholesterol to cells in order to raise the intracellular cholesterol concentration and confirm the protein expression of SREBP2. I didn't know if I should dissolve...

14 January 2021 1,146 6 View

Could you tell me how to inject fluids via tail vein of C57BL6 mouse ?

I am a graduate student in molecular biology in Japan. In the experiment, I am in trouble because I cannot administer the drug from the tail vein of the mouse.Can you tell me how to train mice for...

15 October 2020 2,932 0 View

What does this ghost peak come from?

We analyse oligonucletide using Ion-pair reversed phase chromatography. The mobile phase A is composed of triethylamine (TEA), hexafluoroisopropanol (HFIP), water. And the mobile phase B is the...

27 September 2020 4,434 3 View

HPLC Detector: Evaporative Light Scattering Detector (ELSD)

Dear Colleagues! I am interested in ELSD, an HPLC detector. Is there anyone who is currently using or has used this detector? I would appreciate it if you could share information on the...

01 January 1970 416 14 View

Is it possible to grow terrestrial orchids hydroponically?

Hello everyone. I am curious whether it is possible to grow terrestrial orchids hydroponically under normal conditions, not sterile. Does anyone have such knowledge? I would wonder if Calanthe...

01 January 1970 8,903 10 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

John Tainer

The best approach is to look for functional motifs associated with active site features and activity. Try using a tool like psi blast - http://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE=Proteins&PROGRAM=blastp&RUN_PSIBLAST=on

Nuno Rosa

You can also use ScanProsite tool (http://prosite.expasy.org/scanprosite/ ) to scan for a specific motif.

Other tools you may use are:

http://supfam.cs.bris.ac.uk/SUPERFAMILY/

http://smart.embl-heidelberg.de/smart/set_mode.cgi?NORMAL=1

PS. you can try EnzymeDetector from BRENDA (http://edbs.tu-bs.de/)

Bo Pilgaard

Try PeptidePatternRecognition

http://vbn.aau.dk/en/publications/peptide-pattern-recognition(1400c5df-fa69-4701-8d67-ec5c38cc963b).html