How can we use manganite for Spintronic applications?

How can we use magneto-resistive materials for different applications?Which property is most useful and how temperature coefficient of resistance and magneto-resistance make them useful for bolometry?

06 July 2020 4,485 3 View

Can someone help with my problem with silver staining?

I performed silver staining using a standard protocol. But the problem is As soon as I add silver nitrate to gel, the solution turns white and the gel becomes white. What could be the reason for...

14 September 2015 8,053 4 View

Can anyone provide protocol for catalase assay using total protein?

08 January 2015 6,187 4 View

Why are bands disappeared after staining in SDS-PAGE?

I ran a 15% SDS-PAGE gel. After the end of the run the lower bands of the marker/ladder were visible clearly. But when the gel was stained with commassie stain, the lower half of the bands in the...

02 September 2014 1,511 8 View

Expression of membrane protein

I am trying to express a plant membrane protein using pGEX vector in BL21 strain. I could express the protein but all went into the pellet fraction. Should I purify the protein from the pellet...

12 February 2013 5,570 13 View

Can anyone tell me if it's possible to modify mbSUS (mating based split ubiquitin system) to generate and screen cDNA library?

mbSUS has been used successfully by several researchers to screen for protein-protein interaction for membrane proteins but I could not find if anyone has used it to screen cDNA library. if it is...

06 November 2012 6,244 1 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View