As i tried to grow vibrio using LB agar media, and the whole lawn of vibrio was there after the incubation of 12 hrs at 37 degree. And the colonies were overlapping. Suggest the way to get clear colonies on the plates.
Robert John Wolff
If you are trying to isolate colonies on the plate it appears that your original inoculum has too high of a density. Dilute it in half, and then half again, and maybe again and then do your pour plate or streak for isolation so that you can get isolated colonies for your pure cultures.
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