How can I measure the stomata aperture of the same stomata many times with respect to time after applying some treatments?
Maybe use scotch tape, if feasible for your specimen, place along a small area and when you lift it up you will have a pattern of venation including stomata. This can then be placed on a slide (hemocytometer), or one with a micrometer or grid like on the hemocytometer. Otherwise, I have used a thin plastic ruler placed on top of the slide to estimate the size based on magnification and approximating length between mm bars.
An easy method is imprint with nail varnish. You apply nail varnish on the bottom side of the leaf. Then take off the 'rubber' and measure the stomata size under a microscope :)
I can confirm that the technique described by Andrew works really well - this paper is potentially useful
https://www.researchgate.net/publication/318213918_Changes_in_Stomatal_Density_over_Time_1769-2015_in_the_New_Zealand_Endemic_Tree_Corynocarpus_laevigatus_J_R_Forst_G_Forst_Corynocarpaceae
I also found the Technic described by Andrew very practical. it really works well.
The nail varnish is definitely an option. That will result on a negative imprint from the leaf. I prefer using dental resin that is applied on the leaf and then apply the nail varnish on the resin. In this case you end up with a positive imprint of the leaf. Also once the dental resin is harden you can store it and therefore carry out the imaging/analysis of stomata at a later time, and this can be useful if you collect specimens from outside of lab. Check our paper describing the method
Article Stomatal clustering in Begonia associates with the kinetics ...
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