I have greenish-blue solution (from CuSO4 and bacterial supernatent), and I wont to make sure (by UV) if there are nanoparticles in this solution. How can I know, and what is expected range of nanometers ?
you may find the answer from the following article:
Dang, Thi My Dung, Thi Tuyet Thu Le, Eric Fribourg-Blanc, and Mau Chien Dang. "Synthesis and optical properties of copper nanoparticles prepared by a chemical reduction method." Advances in Natural Sciences: Nanoscience and Nanotechnology 2, no. 1 (2011): 015009.
Of course you can. In a common UV-Vis-NIR spectrophotometer, absorbance spectra is obtained by varying the wavelength of incident radiation, that is inflicted on a dispersion or solution of nanoparticles.
Depending on the nature of material, absorbance properties at different wavelengths show up.
Your solution is coloured means, it has certain absorbance properties in the visible region. But, it does not mean, there are no absorbance in UV-region.
You have to simply acquire data for the entire regime of interest. If it has UV-absorbance characteristics, it'll show up. Don't think about, whether the sample is coloured or not. Simply follow the standard procedure.
1. We can measure the optical absorbance response of solution containing nano particles via wavelength scan (to look for wavelength at which maximum amount of light would be absorbed ( λ max) using UV-visible spectrophotometer).
2. Since, the solution is colored, the λ max would be expected in visible regions (400-800 nm). Also if the solution is very much concentrated or highly colored (darker the color), the absorption response may go out of range, so make sure that you record the absorption response by diluting the solution with appropriate solvent. If you want any more clarity, you could write back to me @ [email protected] or approach via research gate. Good luck.