Well, if you just want improve the quantity, you would fractionate or change the solubility with isoelectric point of zein but this protein has not a good biological value; is very poor in LYS and another esential aminoacids, and its digestibility is poor too. 

If the problem is on solubility, try to use hydrocolloids. However, you do need optimization. Take care.

thanks sir.

What is the best method for isolation of a piperidine alkaloids from the defatted extract of plant

You can use ethyl-alcohol and optimizing by add glycerine for solubulity.

thanks. I will try it.