I want to isolate bacteria from mixed broth culture. Temperature should be 65 C degrees because at lower temperature there is no growth. Agar won't stay solid at this degree so I need to use a broth based technique.
Recently, Russian government officials made recommendations regarding the administration of the COVID-19 vaccine (Sputnik V), which was developed by Russia, in individuals with alcohol addiction....
30 December 2020 6,804 5 View
In fluid dynamics, Bernoulli's principle states that an increase in the speed of a fluid occurs simultaneously with a decrease in static pressure or a decrease in the fluid's potential energy....
18 May 2020 2,538 26 View
I need the general expressions for /nabla levi-civita connection in usual sasakian structure. For ex /(nablaX)Y=
03 December 2019 1,239 0 View
Dear Researchers, I wanted to know whether this model is designed by yours,any software or taken from solidworks tool? Because, accuracy of teeth geometry is crucial. Can you inform me please...
11 March 2019 1,097 8 View
We all know one of the most widely studied phenomena in condensed-matter physics is Kondo effect. Very briefly the milestones are as follows: This field began in 1933 when Wander Johannes de Haas...
19 November 2018 2,023 4 View
Physical mechanism underlying the relation between t, J and U, then the questions are as follows: For the 1/2 filling: When ε_{d} is in between -U and 0, we are in the local moment (intermediate)...
23 January 2017 7,470 2 View
In Slave-Boson formalism we defineci+=bifi+then this yields,=~with a constrain Σ(fifj++bibj)=1where =δ.- Does δ physically...
13 January 2017 9,227 2 View
For the usual doping process, within the resonable bonds we can say that the value of oxygen excess is not very well established, but the ambient highest Tc suggests optimal doping. Remember the...
01 January 2017 6,423 2 View
Is the process MBZ folding just engaged with the AF order?
01 January 2017 8,963 3 View
how can i solve eliiptic problem with fem and mesless method ?
10 October 2015 1,279 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View