Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

HT22 purchase in Greece?

Hello everybody, I am looking for a specific cell line HT22 but I haven't found any company that could import it in Greece. Has anyone faced that problem before? Is there any alternative way that...

01 April 2019 1,917 3 View

Hello, Can anyone help me use krona tool for visualising my NGS data?

My files contain data in percentages or in counts.Does Krona tool need bioinformatic language to be used? Thanks in advance.

27 January 2019 1,758 2 View

Why do I need to wash the entanol from ethanol stored samples with Tris-HCl before extracting DNA?

I worked at a laboratory and before extracting DNA, I washed of the ethanol with Tris-HCl, in order to get rid of all the ethanol before starting the extraction process, but I did not understand...

02 February 2018 9,725 1 View

Yeast fermentation medium recipe??

Can anyone please explain what is a fermentaion medium and a yeast suspension and how to make them? I have attached the paper that these two are mentioned and I have highlighted them in the...

03 January 2018 3,151 2 View

How can i dilute carvacrol (oil) ?

I have read in some papers that carvacrol can be diluted in Tween 80 (1%) and distilled water. I have tried this but carvacrol wasnt fully dissolved. After that i tried to dissolve carvacrol in...

09 August 2016 6,070 7 View

How I can get the Organizational Commitment Questionnaire?

Dear Dr. Meyer, I would like to inform you that I am a student candidate for a PhD in Cyprus University of Technology and my subject relates to job satisfaction, leadership style and commitment of...

27 March 2016 6,828 15 View

Is a standard curve required for each assay when we use an elisa kit?

Does anybody know if i can use some wells ( about 10 wells)of the TNF-a elisa kit in order to try my samples? I have not used a kit before and i wonder whether i can use some wells without running...

25 March 2016 5,274 17 View

Has anyone access to the Emotional Self-awareness questionnaire?

I am looking for a questionnaire which measures the emotional awareness, in other words if it is easy for someone to identify his feelings.So, if you don't have this specific questionnaire, but...

22 March 2016 4,095 2 View

What kind of cells should I to use in order to study the process of inflammation ?

30 November 2015 7,494 12 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View