I identified 11 Mlo gene members in Brachypodium genome by usning bioinformatic tools and now I want to predict those genes, in which tissues types, are expressed.
You can only predict the copy numbers of these genes in the tissue, but not the expression levels. To predict the copy numbers, perform BLAST search with NCBI nr database. With appropriate cut off, filter the blast output. Now, for each NCBI protein id, fetch the metadata, coz, in it, is mentioned, from which tissue, the protein was isolated. I hope this helps.
Thank you very much for your consideration and corecting my question. Could you please send a links or provide a bit detailed steps because I had difficulty to predict, if it is possible?