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Questions related from Abdugaffar Ablazov
I am doing Hygromycin selection of T1 seeds of Transformed Rice Plants (35S-intrest gene). I grew 60 seeds in 50 mg/L of Hygromycin selection media and after one week, I observed very clear 3:1...
13 January 2020 4,412 0 View
I have difficulty to chose the right vector to anayze my interest gene tissue expression in Rice. I have two candidate vectors, such as pCambia1391z and pMpGWB104. Both vectors contain C-terminal...
30 December 2018 8,680 3 View
Previously, I isolated total RNA several times from gram positive bacteria using Trizol reagent. Unfortunately, I obtained degraded RNA each time. I will use extracted RNA for transcriptome...
24 September 2015 1,745 2 View
I identified 11 Mlo gene members in Brachypodium genome by usning bioinformatic tools and now I want to predict those genes, in which tissues types, are expressed.
16 December 2014 1,382 3 View
I want to see whether my identified gene members are produced through gene duplication and what kind of duplication happened.
04 December 2014 5,812 7 View
I want to ligate two different adapters into two different RNA populations and make them hybridize. Please share your ideas regarding to this issue.
28 May 2014 2,852 3 View
As I know bacteria does not contain so much mRNA and so I was planing to use also random hexomers for remaining RNAs but I am not sure the ratio of primers for 2µg/µlRNA ? Please share your...
06 May 2014 3,360 13 View