Abdugaffar Ablazov

7 Questions 10 Answers 0 Followers

Questions related from Abdugaffar Ablazov

Do Homozygote lines grow better than heterozygote lines in Hygromycin selction media?

I am doing Hygromycin selection of T1 seeds of Transformed Rice Plants (35S-intrest gene). I grew 60 seeds in 50 mg/L of Hygromycin selection media and after one week, I observed very clear 3:1...

13 January 2020 4,412 0 View

What is the best binary vector for promoter analysis in Rice?

I have difficulty to chose the right vector to anayze my interest gene tissue expression in Rice. I have two candidate vectors, such as pCambia1391z and pMpGWB104. Both vectors contain C-terminal...

30 December 2018 8,680 3 View

Can someone offer me RNA isolation protocol and Kit from Gram positive bacteria?

Previously, I isolated total RNA several times from gram positive bacteria using Trizol reagent. Unfortunately, I obtained degraded RNA each time. I will use extracted RNA for transcriptome...

24 September 2015 1,745 2 View

How can we demonstrate tisue specific copy number of certain gene members using NCBI database?

I identified 11 Mlo gene members in Brachypodium genome by usning bioinformatic tools and now I want to predict those genes, in which tissues types, are expressed.

16 December 2014 1,382 3 View

What kind of bioinformatic tools can I use for determining gene duplication among Mlo gene members that were identified in tomato?

I want to see whether my identified gene members are produced through gene duplication and what kind of duplication happened.

04 December 2014 5,812 7 View

Is it possible to design an adapter and ligate it to RNA?

I want to ligate two different adapters into two different RNA populations and make them hybridize. Please share your ideas regarding to this issue.

28 May 2014 2,852 3 View

Can I use two different primer such as oligo(dT) and random hexomer at the same time to synthesise cDNA from bacterial total RNA?

As I know bacteria does not contain so much mRNA and so I was planing to use also random hexomers for remaining RNAs but I am not sure the ratio of primers for 2µg/µlRNA ? Please share your...

06 May 2014 3,360 13 View