Dear Abdul Main Although plant tissue and cell culture is performed in aseptic conditions, sometimes contamination occurs. Please go through the links given below; hope these could be helpful to you:

https://www.researchgate.net/post/How_to_control_bacterial_infection_in_a_plant_tissue_culture

https://www.researchgate.net/post/How_to_control_fungal_infection_in_plant_tissue_culture

https://www.researchgate.net/post/Getting_rid_of_bacterial_contamination_in_tissue_culture

In addition, a similar question was put up earlier at RG; hope, answers to that related question might also be helpful to you:

https://www.researchgate.net/post/How-to-prevent-the-microbial-contamination-in-plant-tissue-and-cell-culture

Best wishes, AKC

The article entitled Major Fungal and Bacterial Diseases of Potato and their Management that you get through the link: https://link.springer.com/chapter/10.1007%2F0-306-48575-3_6 can help you get reasonable information for your question.

Thank you!

Not only potato but all types of tissue culture techniques need highly aseptic conditions otherwise infections happen. Infections can happen in any steps of the activities you do for tissue culture. So you should be careful and make more attention to hygiene. You have to sterilize all the equipment before use. Even you have to sterilize equipment in every step as for example you insert potato issue in culture medium through forceps, after doing that sterilize it before using it to the next tissue. In addition, you should use a special isolation chamber for doing all the activities. Always remember it, the more you follow the rules of hygiene, the fewer infections happen.

Phostrol a phosphoric acid derivatives is applied to the foliage for protecting tubers from diseases like pink rot and late blight.

This contamination can be attributed to inadequate disinfectant solution, Contacting the pliers to the glass and endogenous contamination and so on.

by coating and pelleting