Hi Atheer,

You can calculate a therotical pI and Mw from ExPASy software.

Here is your result,

Theoretical pI/Mw (average) for the user-entered sequence:

PCRVER

Theoretical pI/Mw: 8.68 / 758.89.

There is a peptide calculator in the form of an excel sheet here,

spdbv.vital-it.ch/TheMolecularLevel/Goodies/PeptChg.xls

This allows you to put in the number of each amino acid in third column on left and the graph will autocorrect net charges for your particular peptide. I presume this is an internally sequenced fragment so place 0 at N and C terminus values and type in the number of each amino acid as above.

Hope this helps,

Katrina.

At http://pepcalc.com/ you can find MW = 758,89 (as given by Katarina) and pI = 9.29;

The calculator can also present charge to pH plot and hydropathy plot. I use it very often. There is also one more that I could recommend:

http://www.bachem.com/service-support/peptide-calculator/

From the charge to pH plot it comes that at pH 14 the charge is -3.

Hi!

Here is the link for the Expasy website:

http://web.expasy.org/compute_pi/