How can we characterise bacterial transporters ?? probably involved in drug transport ??

26 May 2020 0 7K Report

I am working on a bacterial hypothetical protein, which belongs to a drug transporter family but other than that nothing is know about this portein. I want to characterise it, I have made a knockout already, can anyone help with suggesting some transporter assays or drug transport assays ?

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Tanuka Sen's questions See All

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

Hi, could anyone suggest any mammalian expression plasmid that has a GFP as a selection marker?

I am looking for a plasmid that can express my gene of interest without GFP fusion. But I want its selection marker like neomycin replaced by GFP or any other fluorescent protein so that I can...

29 October 2020 2,247 3 View

Predict charge on a compound at different pH?

Dear fellow scientists, Do you know of a software or a resource base which can predict the charge on the analyte at different pH conditions? Thank you, Abhishek P.S. I am new to HPLC, even a...

19 October 2020 8,605 3 View

How to define Darcy's flow for pore pressure cohesive element in Abaqus?

Hi everyone, I am trying to define a Darcy's flow for pore pressure cohesive element in Abaqus. When I give permeability to the elements, the output of tangential flow rate (GFVR) is always shown...

11 October 2020 1,195 3 View

What is the model equation between resistivity and temperature of a extrinsic semiconductor?

Can anyone Tell me what is the model relation between resistivity and the temperature for a extrinsic semiconductor. Kindly provide with summarized clarification & model equation between...

30 August 2020 6,182 11 View

What is timely Supply chain management issues to be researched as a result of the COVID 19 pandemic?

Dear Research Scholars, Would you please share your opinion on "What is timely Supply chain management issues to be researched as a result of the COVID 19 pandemic?" Thanks Shuvro Sen

22 July 2020 6,995 15 View

Does anyone have a case study of kick/influx and fluid losses during drilling, while MPD (managed pressure drilling) system is active?

Looking for a case study where severe influx and loss events were experienced even when a MPD system is installed and active. Interested in the sequence of events and mitigation steps. A real well...

12 June 2020 8,922 3 View

My SDS gel takes 4-5 hours to run completely. Can someone please help me understand what might be going wrong?

I am performing DNA-Protein Binding assay using Dyna beads. In order to check the bound protein i have to run SDS PAGE. the problem I am facing is my gel takes 4-5 hours to run completely. I have...

09 June 2020 7,358 1 View

Large sample sizes and parametric or non-parametric tests?

I have been reading several sources mentioning that when you have large enough sample sizes (some say 20, others above 200?) then parametric tests, such as t-test, are robust against non-normality...

07 June 2020 3,354 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View