Dear Rakeeb
I am not 100% sure that I understand rightly what you mean with authentication.
Do you mean that you want to make sure that your DNA-Barcode is species specific. In General we can never exclude the possibility that a Barcode is shared by two sibling species, but if you have no indication for two species sharing the same Barcode it is best to assume that it is a single species).
If you on the other hand address the possibility of misidentification of your species, the only Point which can give you some confidence there is a well established reference data base (e.g. BOLD) with all possible species being barcoded (whe are far away from this Situation for sure in nematodes). In this case the barcoding Approach with vouchering the specimen of which you have sequenced the Barcode allows for verifiction of your Determination by other specialists.
But maybe I missed your Problem
Greetings
Nikola
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