I am willing to observe for host cell mRNA expression following viral infection. For that I want to preserve infected cells for future?
A similar question was asked already, but there are RNA-storage buffers for tissue/cell preservation of RNA, and RNAse-free water is a must of course.
https://www.researchgate.net/post/What_is_the_best_way_to_store_RNA
So kind of you Louis but I don't want to extract RNA immediately. So, I need to preserve the infected cells. How can I preserve the cells and extract RNA later?
Oh that's a bit harder... I don't know how many cells with intact RNAs you'll preserve, but you can easily figure that out.
But, you can try a DMSO:FBS (or other stabilizer) formulation (I don't know exactly, a general cell literature database search on the line you're working on should do the trick) to preserve live cells and put them into cryotubes for freezing in a -80ºC alcohol minibath O/N then LN2 for longer-term freezing (or just -80ºC for relatively short term). Just make sure it's very clearly marked as biohazardous if it's a [human] pathogenic virus, and in a separate box!
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