Prem Prasad Lamichhane

6 Questions 8 Answers 0 Followers

Questions related from Prem Prasad Lamichhane

What is the best stimulus for cytokine production by U937 cells?

I tried PMA + Ionomycin (conc 1ug/mL) and PHA (4 ug/mL) stimulation for 4Hrs. It didn't work. Any suggestions and ideas are welcomed.

03 July 2015 4,525 4 View

Does anyone have experience in using pre-designed prime and probes for real-time analysis?

I am planning for gene expression analysis in infected human cell lines. I think using commercial primers and probes for IFNs and ISGs will save time. So, looking for companies that provide best...

08 April 2015 3,333 1 View

How can I dissociate primary dendritic cells for flow cytometry?

I am going to perform flowcytometry of dendritic cells cultured from PBMC. How can I dissociate the adhered cells? Can we trypsinize? How much percentage trypsin is required? Is there any...

27 November 2014 5,317 9 View

Which equilibration buffer be used to equilibrate protein cleanup spin column of AllPrep RNA/Protein kit (Qiagen)?

They didn't supply the equilibration buffer. However I need to equilibrate the column so which buffer could be used? I am planning to do western blot.

01 October 2014 7,141 2 View

How can virus infected cells be preserved for cellular mRNA extraction?

I am willing to observe for host cell mRNA expression following viral infection. For that I want to preserve infected cells for future?

24 September 2014 4,092 3 View

Does TPCK trypsin concentration have an effect on size of plaque?

For the first time I got larger plaques for PR8 while later i got smaller plaques. The TPCK trypsin I used were prepared at different times.

04 September 2014 3,500 3 View