Prem Prasad Lamichhane

2 Questions 6 Answers 0 Followers

Questions related from Prem Prasad Lamichhane

How can I dissociate primary dendritic cells for flow cytometry?

I am going to perform flowcytometry of dendritic cells cultured from PBMC. How can I dissociate the adhered cells? Can we trypsinize? How much percentage trypsin is required? Is there any...

27 November 2014 5,249 9 View

Which equilibration buffer be used to equilibrate protein cleanup spin column of AllPrep RNA/Protein kit (Qiagen)?

They didn't supply the equilibration buffer. However I need to equilibrate the column so which buffer could be used? I am planning to do western blot.

01 October 2014 7,019 2 View