Prem Prasad Lamichhane

4 Questions 7 Answers 0 Followers

Questions related from Prem Prasad Lamichhane

How can I dissociate primary dendritic cells for flow cytometry?

I am going to perform flowcytometry of dendritic cells cultured from PBMC. How can I dissociate the adhered cells? Can we trypsinize? How much percentage trypsin is required? Is there any...

27 November 2014 5,258 9 View

Which equilibration buffer be used to equilibrate protein cleanup spin column of AllPrep RNA/Protein kit (Qiagen)?

They didn't supply the equilibration buffer. However I need to equilibrate the column so which buffer could be used? I am planning to do western blot.

01 October 2014 7,040 2 View

How can virus infected cells be preserved for cellular mRNA extraction?

I am willing to observe for host cell mRNA expression following viral infection. For that I want to preserve infected cells for future?

24 September 2014 4,049 3 View

Does TPCK trypsin concentration have an effect on size of plaque?

For the first time I got larger plaques for PR8 while later i got smaller plaques. The TPCK trypsin I used were prepared at different times.

04 September 2014 3,463 3 View