Pls advise if its ok to use common DNA Extraction kits for tissues to extract total DNA from fish fins, targeting to amplify a gene from mtDNA?

i need some advice on DNA extraction using kits

01 February 2018 1,503 4 View

Why the results presented on the two-way ANOVA table and plot is inconsistent?

I'm trying to perform two-way ANOVA to test the significance of an interaction between treatment (raw/ cooked) and proportion (11 proportions) on moisture content. I used SPSS to generate the...

04 May 2016 5,328 11 View

Is it acceptable to use paired t-test/ Wilcoxon signed-rank in comparing the sensory acceptability of native chicken vs. broiler?

we want to compare the sensory acceptability of native vs. broiler chicken meat in terms of taste, texture, etc. samples of meat were only acquired from the local market, i.e., this is not a...

04 May 2016 6,385 5 View

Aside from DGGE, what other protocols are available for characterization of gut microbiota?

For my dissertation.

07 August 2014 8,424 0 View

Can anyone help with building an isolation room for experimental studies using pigs?

Can anyone provide a detailed instruction on how to build or design an experimental isolation unit? I'm interested in doing an animal model using pigs to test my hypothesis on the pathogenicity of...

07 August 2014 5,553 7 View

Are there existing LAMP-assays for Blastocystis spp., Ascaris spp., and Trichiuris spp.?

I need the assay for my dissertation

06 July 2014 795 1 View

Is there any commercially available fluorescently-labeled monoclonal antibody against E. histolytica? If so, what's the brand name of the manufacturer? thanks

commercially available IFA kit for E. histolytica cysts

10 November 2012 2,645 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View