There is no universal method for extraction of unknown compounds from the biological sample. If you are going to isolation, purification and characterization of non-target compounds you should follow successive extraction (solvents of increasing polarity from a non-polar (hexane) to a more polar solvent (water)) with bioassay-guided. Then you can select the best exaction solvent or solvent mixture according to your purpose. I think as a scientist you have to develop and modify solvent system extract, isolate and purification of your active ingredient with basic of science