A few protocols I have found suggest plating the cells in the presence of siRNA and transfection reagent but I want to knockdown the protein after spheroids have formed. Are there any transfection reagents for this purpose?
Thank you Filippos, where do I get the pLKO.1 TET-ON vector and are there companies that will insert the siRNA for me?
Thank you I found that Dharmacon can generate this for me!
sigma
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