Nancy Krucher

4 Questions 7 Answers 0 Followers

Questions related from Nancy Krucher

Does an increase in reactive oxygen species (ROS) indicate apoptosis?

Our combination treatments cause a reduction in cell number and a 2 fold increase in ROS but our other experiments do not suggest apoptosis: no cleavage of Parp, or increase in Bad expression by...

10 October 2019 8,805 8 View

Easiest way to do invasion assays?

What kit is best and easiest?

05 May 2019 6,546 4 View

What is the best kit for measuring invasion in pancreatic cancer cells?

using Panc1 and MiaPaCa2 cells

03 March 2017 1,417 2 View

How can one use siRNA to knockdown a gene in an already formed breast cancer spheroid growing in Matrigel?

A few protocols I have found suggest plating the cells in the presence of siRNA and transfection reagent but I want to knockdown the protein after spheroids have formed. Are there any transfection...

10 October 2014 2,998 3 View