How can one get enough volume of blood that will give sufficient plasma or serum from an experimental mice for biochemical assay?

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Patriotisme ou Nationalisme économique ?

Patriotisme et Nationalisme économique Le patriotisme économique promeut une politique industrielle qui sort du modèle classique et redéfinie les relations socio-économiques entre les nations,...

20 June 2024 6,118 1 View

How do you add a new Institution to ResearchGate?

How do new institutions become listed on ResearchGate, please? Where can you add the logo and details for other researchers to find your university or college?

30 May 2024 5,836 2 View

Est-il possible de penser que le capitalisme est en déclin avec la motée du nationalisme économique?

De plus en plus il est observé dans divers coins du monde un retour au nationalisme économique. Des revendications multiples fusent des politiques et même des populations sur les habitudes de...

29 May 2024 5,156 4 View

Salut, La conférence pourrait-elle développer les questions de culture financière ?

Pour l'Afrique centrale d’où je suis les populations restent en marge des activités économiques formelles qui impulserait le développement du fait du manque de culture financière. Un marché...

29 May 2024 5,154 0 View

I need EEG data set for seizures, sleep, awake and epilepsy. In which open source i can access the data?

Electroencephalography (EEG) data set for awake, sleep, and seizures

23 May 2024 7,148 0 View

How can we integrate indigenous knowledge with scientific methods for sustainable local food systems?

How can farmers integrate indigenous knowledge with scientific methods for sustainable local food systems?

19 May 2024 6,038 1 View

Balancing food sovereignty, sustainability and economic development in local food systems?

How can local communities and local government balance food sovereignty, sustainability and economic development in local food systems?

19 May 2024 244 2 View

Governance mechanisms to empower communities in autonomous food system management?

How can governance mechanisms be utilized effectively to empower communities in autonomous food system management?

19 May 2024 5,755 4 View

Magnesium Hydrogenation process ?

I have recently begun working with magnesium powder, looking at the de/hydrogenation thermodynamics and kinetics using a Hiden Isochema IGA-003 (Gravimetric gas sorption). What I would like to...

08 May 2024 4,107 0 View

What can cause drifting counts per second measurements in ICP-MS ?

In my icpms analysis there are 4 measurements taken for each sample. The counts per second for some elements, especially Iron, drifts downward for each of the 4 measurements. For example one...

11 April 2024 3,083 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

How can rhizobial strains be evaluated without plant experiments?

Hi everyone, I'm looking for a method to evaluate the nodulation ability and effectiveness of different strains of rhizobia. Is there any method that avoids testing in plants? Are there any...

17 July 2024 7,249 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View