I need to get primers for a large set of ORFs specific of a taxon (Eubacteria; taxid:2)). I know can get the primers from the BatchPrimer3, but the mispriming libraries offered at http://probes.pw.usda.gov/cgi-bin/batchprimer3/batchprimer3.cgi are only for plant species. I know I can get the primers and the specificity against all bacterial nuclotide sequences deposited with the primer BLAST application, but this application does not support batch entries and I have over 500 ORFs...

Similar questions and discussions