Dear Wei,

Please see the paper I have attached below. It may help you.

cancerres.aacrjournals.org/content/suppl/2008/10/07/68.../smethods.pdfSimilar

Thank you, Sudip.

Wei

hi Deng,

usually we use the formula as

tumor volume= lengthX breadth^2X 0.52.

This is the modified formula for ellipsoid that we use to calculate tumor volume in xenograft implants in mice. you can see my paper that was published last year in Endocrinology.

hope this helps.

best

Khalid Masoodi

Thank you, Khalid.

Wei

Hi !

I would like to understand the relation between TGI and T/C ratio. I know it's 2 different things but on all my researches, these two terms are very close.

Thank you !

Yael

I am a bit late to comment on this, but the TGI is a tricky question.

There are many ways to define it. But I would typically express it as: TGI(%) = (Vc-Vt)/(Vc-Vo)*100, where Vc,Vt are the median of control and treated groups at the end of the study and Vo at the start.

In terms of the tumour volume, Khalid suggested the calculation of a spheroid, which implies major assuptions in tumour morphology. This is the main method to estimate subcutaneous tumour volume, however, it massively overestimates volumes. I recommend to apply the formula length*width*height/2, which is more precise. Also, if interested, have a look at this: http://lan.sagepub.com/content/early/2014/12/05/0023677214562653.1.abstract

Hope to have helped

Dear Juan A Delgado San Martin Please guide

How we can calculate tumor metastasis index ?

Juan's answer seems pretty standard to me:

TGI = (Vc1-Vt1)/(Vc0-Vt0)

Vc1 and Vt1 are the mean volumes of control and treated groups at time of tumor extraction, while Vc0 and Vt0 are the same groups at the start of dosages.

I know there may be some other statistical tools to compare control and treatment groups, maybe use a student's t-test to determine significance levels if you have just two groups you're comparing. This list of xenografts (http://altogenlabs.com/xenograft-models/) has accompanying charts/statistics so you can see what reasonable values might look like.

Dear Matt,

could you please provide a reference paper for the above formula please, which I can cite?

Thanks!

Tumor growth inhibition rate (%); can be calculated using the following formula: = (Vc1-Vt1)/(Vc0-Vt0) X 100

where, Vc1 is the mean tumor volume in the control group at the time of tumor extraction, and Vt1 represents the mean tumor volume in the treatment groups at the time of tumor extraction, respectively. Whereas, Vc0 and Vt0 are the mean tumor volumes in the control group and treatment group at the beginning of the experiment, respectively.

%TGI = [(1-(Vt1/Vto)/(Cto/Ct1))/(1-Cto/Ct1)]*100.

And try to use tumor volumes when the tumor height is closer to the tumor width, and when the shape of the tumor closer to the ellipsoid shape to avoid large differences between the real and the calculated volumes due to assumptions one makes in 2-dimensional caliper measurements.