When I run a O2 and N2 standard in the gas chromatograph, the peaks are overlapped, How can I improve the resolution of the chromatogram. This situation is new, last monththe calibrationwent just fine...thanks
Hi
You have many parameters to adjust to improve the resolution on GC (decreasing flow rate,ramp temperature,increase split ratio....etc)
Hope the attached link below will be useful
https://www.google.com/amp/s/www.researchgate.net/post/How_can_I_improve_the_resolution_of_the_peaks_in_gas_chromatography/amp
I suggest that you confirm your instrument is running properly, no leaks are present, the temperatures are correct, gas flows set correctly, and you are injecting the correct amount of sample (not overloading the column). Inject a standard to verify column performance. IF everything appears to be OK, but your standard(s) peak shape and retention are off..... then....perhaps you have fouled your GC column? Follow the manufacturer's advice regarding cleaning of the GC column and/or replace the column.
William Letter I think that the problem must be the column, all the parameters are ok, and the GC uses a sample loop, so I should not have any problem with column overload I think...All seems to be column issues, I will follow your advice and check with the manufacturer...thanks, I really appreciate
Grzegorz Boczkaj I use a Hayesep Q 80/100Mesh 1m x 1/8¨ IS and a Molsieve 5A80/100 Mesh also 1m x 1/8¨ IS columns
So, Porapak Q (Hayesep same) is not recommended for O2 and N2 separation.
Molesieve 5A should work well. If it is not you need to condition it at 250 or even 300 deg. C for several hours and it stary to separate well. If you don't need to detrrmine N2 you can also use N2 as a carrier and reference gas in TCD, this way you will not see it on the chromatogram.
Regards
GB
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