Im trying to validate mass photometry for full/empty ratio determination but since there are no good reference standard material available how can i do this?
In the absence of standard reference materials, validating analytical techniques for rAAV characterization, such as mass photometry for full/empty ratio determination, requires alternative approaches. Strategies include creating in-house reference materials, conducting comparative analysis with established techniques, performing method robustness studies, collaborating with other laboratories, employing matrix-matched calibration, and focusing on critical validation parameters like specificity and accuracy. These approaches collectively enhance confidence in the reliability of the analytical technique despite the lack of commercial standards.
Thank you for the insights. I know about comparing with established techniques like AUC or CDMS but then the question is how are these techniques validated without standards what's the starting point? the same goes with internal reference standard material how do you characterize these without validated techniques? it's this chicken or the egg first scenario that i can't wrap my head around.
When validating analytical techniques without standards, start by reviewing existing literature and leveraging comparative analysis with established methods. Develop in-house reference materials, conduct robustness studies, collaborate with other labs, and focus on critical validation parameters like specificity and accuracy. Despite the lack of standards, these approaches can enhance confidence in the reliability of the analytical technique.
