I would like to use urea as precipitation agent. But, when I used this reagent the solution was not precipitated. The urea concentration was 0.5 mol also more higher concentration.
Precipitation require the condition that ionic product exceed solubility product. So you have to maintain that condition for precipitation.
What is difference between ARIMA and ARMA
30 September 2023 5,557 2 View
Raw material was calcined at 1000oC and sintered at 1400oC. The calcined powder was existed at tetragonal and small amount of monoclinic phase. After sintering, the phase was changed to purely...
18 May 2015 3,695 4 View
I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...
06 August 2024 1,350 4 View
Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...
09 July 2024 5,508 0 View
I synthesize silver nanoparticles ,and reserve it in water. Then, when I sonicate it some minutes or longer ,it couldn't be dispersed, most of them aggregate .Otherwise ,when I sonicate several...
09 July 2024 7,335 0 View
I have a 0.25mM thick gel and I am having trouble cleaning out the wells to ensure constant sample application. I usually use a thin syringe to get into the wells and rinse them out with running...
01 July 2024 2,124 2 View
Some scientists use nano nitrogen and some use nano urea, even though the process used the same for preparation. So, which is true nano nitrogen or nano urea?
28 June 2024 2,885 6 View
Fertilizers play a crucial role in enhancing the productivity of plants. However, low nutrient use efficiencies of conventional fertilizers (CFs) associated with several losses have led to...
21 June 2024 8,843 3 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
Hello, I am trying to purify a 6X-His tagged protein using Ni-NTA chromatography. However, I obtain very less yield & concentration; and considerable protein in load flow through. Any...
16 June 2024 5,876 5 View
Does someone know any precipitating agent to precipitate glucose from 10 % glucose solution? or to remove glucose from solution
13 June 2024 438 0 View
Hello I have synthesized a dipeptide in an aqueous media. Now I want to precipitate and filter it. How to precipitate it. Thank you in advance. Velmurugan S
11 June 2024 525 0 View