How can I use toluene to treat E.coli cells for FITC Staining?

05 January 2016 1 5K Report

To enhance the efficiency of FITC staining, I would like to use toluene as permeability agent. But I can't get the working protocols for E. coli cells. Is there anyone who can explain to me how to use it?

Belay Tekalign

Maybe

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