In which dry organic solvent/solvent mixture molten 1H-imidazole could be precipitated at 100%?

I am trying to isolate an allkylated imidazole from a mixture containing unreacted 1H-imidazole. The problem is that imidazole is slightly soluble in my targed molecule. Furthermore, I need to...

04 November 2020 4,513 3 View

Bioinformatic tools to determine ploidy in microbial genomes?

Looking for tools with proved accuracy to detect ploidy levels in microbial whole-genomes.

14 October 2020 4,273 3 View

What can be causing uneven focus on IHC-P stained slides?

Hello, I have been performing some IHC staining on parafin embeded tissue. All was good until recently. In the latest slides I have prepared, when looking under the microscope, some areas need to...

12 February 2020 9,906 3 View

[COMSOL 5.1] Simple FSI simulation not converging. Can someone please take a look at my model?

Hello, I'm a newbie on CFD and starting to learn how to use COMSOL. I tried to modify one example from COMSOL but it isn't working. I think it has something to do with Inlet velocity, but i...

11 April 2019 2,662 3 View

How can I use FSI+Piezoelectric module in COMSOL Multiphysics?

I am trying to model Piezoelectric effect using vortex induced vibrations on a cylinder attached to a fixed beam while the end of the beam has a piezoelectric patch in 2D, but when I add the...

27 March 2019 6,694 3 View

Why the isotopes tends to concentrate in tumours?

What happens in the cancer cells or diseases to ''attract isotopes''

12 November 2018 7,361 3 View

How to intrepert and report two-way anova + post-hoc analysis data?

I have an animal experiment with 4 different groups: A) control diet + vehicle B) control diet + drug C) experimental diet + vehicle D) experimental diet + drug. We then measure the expression of...

25 June 2018 3,828 3 View

How can I avoid tissue damage during IHC protocol?

Hello! I'm trying to validate CTLA-4 antibody, but until now the only staining I have is 1:50 with heating and enzymatic pre-treatments. However, the tissue is very damaged in the end, even after...

18 December 2017 8,839 5 View

I need to extract total RNA from protoplasts, could anyone help me with a detailed protocol?

22 June 2017 9,578 1 View

In the ChIP-qPCR assays what is the best method for DNA quantification and how should I normalize my data?

I started using ChIP in my analysis a short time ago and I have a lot of questions about how to normalize my data, what percentage of Input should I use and what is the best method to quantify my...

14 June 2017 7,259 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View