How can I use centrifuging to separate mucins from LC-AX?

Florence Van Herreweghen @Florence-Van-Herreweghen

16 April 2014 0 5K Report

For quantification of mucins in my bacterial growth medium I used a fluorometric assay. The problem is that my prebiotics interfere with these measurements. Since there is a big difference in molecular weight: mucins 40*10³ kDa and for example long chain arabinoxylans 20 kDa, I was hoping I could separate them by centrifuging.

The question is: how long and at what speed? They are both dissolved in the medium, and I have no idea about the viscosity of the medium etc. Can anybody help?

Badges
Science topic

More Florence Van Herreweghen's questions See All

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Explain plasmid description?

Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....

14 January 2021 6,326 2 View

Is there a generalized method to derive current-mode model of any DC-DC converters?

Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...

06 January 2021 7,247 3 View

Most appropriate fit of a standard curve in an enzym-linked lectin assay?

Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...

03 January 2021 1,010 6 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

Which Universities are virtual?

Many Universities have employees located across the world.

22 December 2020 6,380 3 View

How to get rid of tailing peaks?

I have been making a method to determine chlorhexidine using an LC-MSMS (ESI+ modus). My solutions are made in acetonitril/water (65/35) and 0,2% formic acid is added to ionize the compound. My...

14 December 2020 3,855 6 View

Sample size fixed effect regression ?

Is it true that only a very large sample is suitable for a panel data fixed effects estimation, or do the ols criteria apply?

26 November 2020 9,127 4 View

How can I prevent gene expression before collecting microorganisms by centrifugation?

Hy everybody, I am looking for a way to stop gene expression during harvesting of microalgae to ensure reliable RNAseq in response to temperature. I am affraid working on ice and performing...

25 November 2020 7,282 3 View

How to increase the efficiency of overexpression using lentiviral TET-ON system in primary kidney cancer cells?

I am using a lentiviral TET-ON system for conditional overexpression of my gene of interest in primary kidney cancer cells. I use viral load from transfection of HEK293T cells after 48 and 72...

16 November 2020 2,496 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

No title

23 February 2021 4,014 3 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

Does viral RNA extraction from nasopharyngeal swabs require pre-centrifugation with supernatant collection?

06 February 2021 323 3 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the minimum centrifugation RPM and minimum time for TiO2 nanoparticle synthesis from plant extract?

In my laboratory, I have an analog centrifuge (does not show real RPM value). Its' brochure indicates the range is 300-3000 RPM. The regulator is marked from 1 to 10. Is there any way to determine...

01 February 2021 2,061 4 View

How to calculate the polysaccharide content of a curcuma species through HPLC method?

what all standards are needed to quantify and analyze the composition with a proper method fie for the mobile phase to run the sample ?

31 January 2021 7,704 4 View

Does anyone have experience with NCI H716 cell line?

Hello! I am working with NCI H716 cells and I am struggling a bit trying to "wash" the cells since they are producing significant debris. I have centrifugated and resuspended them in their media...

28 January 2021 6,421 1 View

How to extract polysaccharids from Aloe gel?

I 'm trying to extract polysacharides from Aloe leaf and tried ethanol percipitation, but after getting the pellet. I used the protocol from following reference following the steps, but I don't...

27 January 2021 7,049 5 View