Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

How should I compute a new variable with z-scores?

I would like to create a new variable using 4 different measures: hIndex (range: 1-47), # of publications (range: 0-111), and 2 other measures (range: 1-7). I changed all of these variables into...

09 February 2021 4,866 3 View

Can cell lines have different DNA content?

I am doing calibration curves using CyQUANT Direct Assay to 10 different pancreatic cancer cell lines. I've noticed that the fluorescence of some cell lines double at the same cell number. Is this...

03 February 2021 3,587 2 View

Have you cloned a PCR product containing 2X2A sequences into a vector that contains another 2A sequence?

Hi! I am having problems cloning a 1.6 kb fragment containing 2 different genes and 2X2A peptides into a vector that I already used to clone other fragments (digested with PmeI/BamHI). The vector...

17 December 2020 5,218 3 View

How to migrate a graph database to SQL?

In the research that I am working on, I need to migrate a graph database to sql to carry out tests, I am using neo4j as a tool, the information I have found is about migrating from SQL to neo4j...

09 November 2020 1,869 3 View

Can I incubate two secondary antibodies at the same time for western blot?

I want to know if I can use a goat-anti mouse IgM-HRP and a goat-anti mouse IgG-HRP to treat a western blot membrane at the same time. Thank you

26 September 2020 1,332 6 View

How to obtain Uranium metal from Uranyl Nitrate?

I got some Uranyl Nitrate crystals and want to get Uranium metal, to start, think I can use heating to decompose the nitrate salt in order to get the oxide, and then I should reduce the oxide to...

22 August 2020 525 3 View

Is it dangerous for me to handle Ethyl methanesulfonate (EMS) in an open air space?

18 June 2020 3,918 3 View

What are the most common data file types used to create Virtual Reality environments?

I know most of the 3D file types used in VR but I could be missing out on some that are more commonly used and would like to see what others are using. Also any ideas on what file types relating...

19 February 2020 3,207 3 View

Is there any free software similar to WinNonlin?

I want to predict a toxicocinetic model of some molecules. I have windows and linux.

17 September 2018 7,412 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View